- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-DNMT1
DNMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat et plus (5)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA
Conjugaison
Non conjugué
N° de cat : 24206-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules C6, cellules HEK-293, cellules HepG2, cellules Jurkat |
Résultats positifs en IP | cellules HEK-293, |
Résultats positifs en IHC | tissu de cancer du poumon humain, tissu de cancer du sein humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HepG2, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 12 publications below |
WB | See 86 publications below |
IHC | See 5 publications below |
IF | See 10 publications below |
CoIP | See 2 publications below |
ChIP | See 5 publications below |
RIP | See 1 publications below |
Informations sur le produit
24206-1-AP cible DNMT1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat
Réactivité | Humain, rat |
Réactivité citée | rat, Chèvre, Humain, poisson-zèbre, porc, poulet, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | DNMT1 Protéine recombinante Ag19116 |
Nom complet | DNA (cytosine-5-)-methyltransferase 1 |
Masse moléculaire calculée | 1632 aa, 185 kDa |
Poids moléculaire observé | 180-200 kDa |
Numéro d’acquisition GenBank | BC126227 |
Symbole du gène | DNMT1 |
Identification du gène (NCBI) | 1786 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)
Protocole
Product Specific Protocols | |
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WB protocol for DNMT1 antibody 24206-1-AP | Download protocol |
IHC protocol for DNMT1 antibody 24206-1-AP | Download protocol |
IF protocol for DNMT1 antibody 24206-1-AP | Download protocol |
IP protocol for DNMT1 antibody 24206-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Environ Int Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels | ||
Cell Death Dis Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis | ||
Chemosphere Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae | ||
Bioact Mater Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.
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Clin Transl Med Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1. | ||
J Transl Med The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Roy (Verified Customer) (09-17-2025) | Great band at the expected size. The dilution used was 1/1000 with an 1h incubation at Room Temperature
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FH Paula (Verified Customer) (12-14-2021) | good bands at the right molecular weight, but a lot of smear
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