Anticorps Polyclonal de lapin anti-DNMT1

• Phare
• Validé par KD/KO

DNMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24206-1-PBS

Synonymes

AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1, DNMT

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Galerie de données de validation

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  WB analysis using 24206-1-AP (same clone as 24206-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24206-1-AP (same clone as 24206-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 24206-1-AP (same clone as 24206-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 24206-1-AP (same clone as 24206-1-PBS)

  IP result of anti-DNMT1 (IP:24206-1-AP, 4ug; Detection:24206-1-AP 1:500) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24206-1-AP (same clone as 24206-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNMT1 antibody (24206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24206-1-PBS cible DNMT1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNMT1 Protéine recombinante Ag19116
• Nom complet: DNA (cytosine-5-)-methyltransferase 1
• Masse moléculaire calculée: 1632 aa, 185 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC126227
• Symbole du gène: DNMT1
• Identification du gène (NCBI): 1786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)

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