Anticorps Monoclonal anti-DNMT1

DNMT1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2G5C5

Avis (1)

N° de cat : 68485-1-Ig

Synonymes

AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, FLJ16293, M.HsaI, MCMT

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Galerie de données de validation

• 

  WB analysis using 68485-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68485-1-Ig (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

68485-1-Ig cible DNMT1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNMT1 Protéine recombinante Ag19116
• Nom complet: DNA (cytosine-5-)-methyltransferase 1
• Masse moléculaire calculée: 1632 aa, 185 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC126227
• Symbole du gène: DNMT1
• Identification du gène (NCBI): 1786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)

Publications

Species	Application	Title
human	WB,IF	Gene; Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database; Authors - Jianjun Wu
mouse	IF	Adv Sci (Weinh); Paternal Obesity-Induced H3K27me3 Elevation Leads to MANF-Mediated Transgenerational Metabolic Dysfunction in Female Offspring; Authors - Yajun Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (04-26-2024)

Works very well in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver and mouse brain

DNMT1 Antibody Western Blot validation (1:1000 dilution) in mouse liver and mouse brain (Cat no:68485-1-Ig)

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