Anticorps Recombinant de lapin anti-DNMT1

DNMT1 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240302E2

N° de cat : 82678-2-PBS

Synonymes

240302E2, AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1

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Galerie de données de validation

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  WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNMT1 Recombinant antibody (82678-2-RR, Clone:240302E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82678-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82678-2-RR against Human DNMT1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82678-2-PBS cible DNMT1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DNMT1 Protéine recombinante Ag29479
• Nom complet: DNA (cytosine-5-)-methyltransferase 1
• Masse moléculaire calculée: 1632 aa, 185 kDa
• Numéro d’acquisition GenBank: BC126227
• Symbole du gène: DNMT1
• Identification du gène (NCBI): 1786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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