Anticorps Polyclonal de lapin anti-DNMT2

• Phare
• Validé par KD/KO

DNMT2 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19221-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 19221-1-AP (same clone as 19221-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 19221-1-AP (same clone as 19221-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 19221-1-AP (same clone as 19221-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 19221-1-AP (same clone as 19221-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19221-1-AP (same clone as 19221-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19221-1-PBS cible DNMT2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNMT2 Protéine recombinante Ag5571
• Nom complet: tRNA aspartic acid methyltransferase 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC047733
• Symbole du gène: DNMT2
• Identification du gène (NCBI): 1787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Dnmt2 was initially assigned a member of the DNA methyltransferase family on the basis of its extensive homology with eukaryotic and prokaryotic DNA-(cytosine C5)-methyltransferases . However, in the apparent absence of a phenotype in dnmt2 knockout cells, Dnmt2's possible biological function remained unknown , even though Dnmt2 is strongly conserved and it is found in species ranging from Schizosaccharomyces pombe to human. Later very weak, residual DNA methylation activity was found with enzymes from different species. The finding that Dnmt2 is an active RNA methyltransferase capable of methylating the C38 position of the tRNA Asp came as a surprise . However, still no cellular function of the tRNA Asp methylation has been found, although in Zebrafish Dnmt2 knock-down caused a developmental phenotype . It is very intriguing that an enzyme that looks like a DNA methyltransferase can methylate RNA, in particular since the RNA and DNA specific m5 C methyltransferases use different catalytic residues and a different mechanism for the methyl transfer reaction . the predicted molecular weight of Dnmt2 is 45 kDa, but it may migrate to the 40 kDa (PMID: 18567810 )

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