Anticorps Polyclonal de lapin anti-DNMT3A

• Phare
• Validé par KD/KO

DNMT3A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

32

N° de cat : 20954-1-AP

Synonymes

DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT3A, DNMT3A2, M.HsaIIIA

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Galerie de données de validation

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  WB analysis using 20954-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20954-1-AP

  WB result of DNMT3A antibody (20954-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNMT3A transfected HepG2 cells.

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  WB analysis using 20954-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20954-1-AP

  IP result of anti-DNMT3A(IP:20954-1-AP, 4ug; Detection:20954-1-AP 1:5000) with HeLa cells lysate 1560 ug.

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  IHC staining of human stomach cancer using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 20954-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of K-562 using 20954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules HepG2, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de côlon de rat, tissu de côlon de souris, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• ChIP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

20954-1-AP cible DNMT3A dans les applications de WB, RIP, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNMT3A Protéine recombinante Ag15111
• Nom complet: DNA (cytosine-5-)-methyltransferase 3 alpha
• Masse moléculaire calculée: 912 aa, 102 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC043617
• Symbole du gène: DNMT3A
• Identification du gène (NCBI): 1788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

Publications

Species	Application	Title
human	WB,ChIP	Mol Cancer; CircNTNG1 inhibits renal cell carcinoma progression via HOXA5-mediated epigenetic silencing of Slug; Authors - Yanping Liang ; KD Validated
human	IF	Sci Adv; Tumor suppressor CEBPA interacts with and inhibits DNMT3A activity.; Authors - Xiufei Chen
mouse	WB	Protein Cell; Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.; Authors - Hongling Zhang
human	ChIP	Cell Biol Toxicol; Hypermethylation of microRNA-497-3p contributes to progression of thyroid cancer through activation of PAK1/β-catenin.; Authors - Yuxia Fan
human	WB	J Mol Cell Cardiol; LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.; Authors - Xinxing Zhu
human	WB	Front Cell Dev Biol; piRNA-823 Is Involved in Cancer Stem Cell Regulation Through Altering DNA Methylation in Association With Luminal Breast Cancer.; Authors - Xin Ding

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