Anticorps Recombinant de lapin anti-DNMT3A

DNMT3A Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242257E1

N° de cat : 81474-5-RR

Synonymes

EC:2.1.1.37, EC:2.1.1.-, DNA MTase HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, Cysteine methyltransferase DNMT3A

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Galerie de données de validation

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  WB analysis using 81474-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81474-5-RR (DNMT3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 81474-5-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 81474-5-RR (DNMT3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81474-5-RR

  Biolayer interferometry (BLl) kinetic assays of 81474-5-RR against Human DNMT3A were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules MCF-7, cellules NIH/3T3, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81474-5-RR cible DNMT3A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DNMT3A Protéine recombinante Ag15111
• Nom complet: DNA (cytosine-5-)-methyltransferase 3 alpha
• Masse moléculaire calculée: 912 aa, 102 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC043617
• Symbole du gène: DNMT3A
• Identification du gène (NCBI): 1788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

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