Anticorps Polyclonal de lapin anti-DNMT3B

DNMT3B Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 26971-1-AP

Synonymes

DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, DNMT3B, ICF, M.HsaIIIB

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Galerie de données de validation

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  WB analysis using 26971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du sein humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

26971-1-AP cible DNMT3B dans les applications de WB, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNMT3B Protéine recombinante Ag25117
• Nom complet: DNA (cytosine-5-)-methyltransferase 3 beta
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 85-96 kDa
• Numéro d’acquisition GenBank: NM_001207055
• Symbole du gène: DNMT3B
• Identification du gène (NCBI): 1789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.

Publications

Species	Application	Title
human,mouse	IF,WB	Protein Cell; The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting; Authors - Yuhan Chen
human	WB	Front Cell Dev Biol; miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis; Authors - Dong Pan
rat	IHC	Andrology; Methylation of eNOS in the rat penile corpus cavernosum under different pathological states and its relationship with erectile function; Authors - Na Wang
human	WB	Brain Sci; Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes.; Authors - Kalaiselvi Sivalingam
human	WB	Biol Res; DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.; Authors - Ying Zhu
human	WB	Integr Cancer Ther; Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.; Authors - Liang Wang