Anticorps Polyclonal de lapin anti-DNMT3B

• Phare
• Validé par KD/KO

DNMT3B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26971-1-PBS

Synonymes

ICF, EC:2.1.1.37, DNMT3, DNMT, DNA (cytosine-5)-methyltransferase 3B

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Galerie de données de validation

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  WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 26971-1-AP (same clone as 26971-1-PBS)

  IP result of anti-DNMT3B (IP:26971-1-AP, 4ug; Detection:26971-1-AP 1:5000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26971-1-PBS cible DNMT3B dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNMT3B Protéine recombinante Ag25117
• Nom complet: DNA (cytosine-5-)-methyltransferase 3 beta
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 85-96 kDa
• Numéro d’acquisition GenBank: NM_001207055
• Symbole du gène: DNMT3B
• Identification du gène (NCBI): 1789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.

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