Anticorps Recombinant de lapin anti-DOCK11

DOCK11 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242575H5

N° de cat : 85022-3-PBS

Synonymes

242575H5, ACG, bB128O4.1, dedicator of cytokinesis 11, ZIZ2

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Galerie de données de validation

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  WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

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  WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

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  WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85022-3-RR (same clone as 85022-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85022-3-RR (DOCK11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85022-3-RR (same clone as 85022-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85022-3-RR (DOCK11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01759-2

  Cytometric bead array standard curve of MP01759-2, DOCK11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85022-3-PBS. Detection antibody: 85022-1-PBS. Standard: Ag35831. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85022-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85022-3-RR against Human DOCK11 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85022-3-PBS cible DOCK11 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DOCK11 Protéine recombinante Ag35831
• Nom complet: dedicator of cytokinesis 11
• Masse moléculaire calculée: 238 kDa
• Poids moléculaire observé: 238 kDa
• Numéro d’acquisition GenBank: NM_144658
• Symbole du gène: DOCK11
• Identification du gène (NCBI): 139818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DOCK11(also known as Zizimin2) is a member of CDM (Ced-5, DOCK180, and Myoblast City) family guanine nucleotide exchange factor mainly expressed in immune cells. As a guanine nucleotide exchange factor, DOCK11 activated the rho family GTPase cell division cycle 42 (CDC42), resulting in cytoskeletal reorganization.

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