Anticorps Monoclonal anti-DOCK2

• Phare
• Validé par KD/KO

DOCK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D7A8

N° de cat : 66969-1-PBS

Synonymes

dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209

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Galerie de données de validation

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  WB analysis of Jurkat using 66969-1-Ig (same clone as 66969-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66969-1-Ig (same clone as 66969-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK2 antibody (66969-1-Ig, Clone: 1D7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66969-1-PBS cible DOCK2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DOCK2 Protéine recombinante Ag28582
• Nom complet: dedicator of cytokinesis 2
• Masse moléculaire calculée: 212 kDa, 38 kDa
• Poids moléculaire observé: 200-210 kDa
• Numéro d’acquisition GenBank: BC016996
• Symbole du gène: DOCK2
• Identification du gène (NCBI): 1794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.

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