Anticorps Polyclonal de lapin anti-DOCK3; MOCA

DOCK3; MOCA Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20683-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20683-1-AP (same clone as 20683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 20683-1-AP (same clone as 20683-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 20683-1-AP (same clone as 20683-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 20683-1-AP (same clone as 20683-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20683-1-AP (same clone as 20683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20683-1-AP (same clone as 20683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20683-1-PBS cible DOCK3; MOCA dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: dedicator of cytokinesis 3
• Masse moléculaire calculée: 233 kDa
• Poids moléculaire observé: 233 kDa
• Numéro d’acquisition GenBank: NM_004947
• Symbole du gène: DOCK3
• Identification du gène (NCBI): 1795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Dedicator of cytokinesis 3 (DOCK3), also named as MOCA and PBP , is a ~180 kDa protein involved in signaling trasduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3β (GSK-3β) at the plasma membrane, thereby promoteing axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS.

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