Anticorps Polyclonal de lapin anti-DOCK5

• Phare
• Validé par KD/KO

DOCK5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 29938-1-AP

Synonymes

dedicator of cytokinesis 5

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Galerie de données de validation

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  WB analysis using 29938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 29938-1-AP

  WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells.

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  WB analysis using 29938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of DU 145 using 29938-1-AP

  IP result of anti-DOCK5 (IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug.

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  IHC staining of mouse kidney using 29938-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 29938-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules DU 145, cellules Jurkat, cellules NIH/3T3, cellules NIH3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules DU 145,
• Résultats positifs en IHC: tissu rénal de souris, human intrahepatic cholangiocarcinoma tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29938-1-AP cible DOCK5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DOCK5 Protéine recombinante Ag32193
• Nom complet: dedicator of cytokinesis 5
• Masse moléculaire calculée: 1870 aa, 215 kDa
• Poids moléculaire observé: 190 kDa
• Numéro d’acquisition GenBank: BC137175
• Symbole du gène: DOCK5
• Identification du gène (NCBI): 80005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.

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