Anticorps Polyclonal de lapin anti-DOCK5

• Phare
• Validé par KD/KO

DOCK5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29938-1-PBS

Synonymes

dedicator of cytokinesis 5

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Galerie de données de validation

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  WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 29938-1-AP (same clone as 29938-1-PBS)

  WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  IP experiment of DU 145 using 29938-1-AP (same clone as 29938-1-PBS)

  IP result of anti-DOCK5 (IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 29938-1-AP (same clone as 29938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP (same clone as 29938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP (same clone as 29938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29938-1-AP (same clone as 29938-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29938-1-PBS cible DOCK5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DOCK5 Protéine recombinante Ag32193
• Nom complet: dedicator of cytokinesis 5
• Masse moléculaire calculée: 1870 aa, 215 kDa
• Poids moléculaire observé: 190 kDa
• Numéro d’acquisition GenBank: BC137175
• Symbole du gène: DOCK5
• Identification du gène (NCBI): 80005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.

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