Anticorps Polyclonal de lapin anti-DOCK9

DOCK9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18987-1-PBS

Synonymes

Zizimin-1, ZIZ1, dedicator of cytokinesis 9, Cdc42 guanine nucleotide exchange factor zizimin-1

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Galerie de données de validation

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  WB analysis of A549 using 18987-1-AP (same clone as 18987-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  WB analysis using 18987-1-AP (same clone as 18987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 18987-1-AP (same clone as 18987-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 18987-1-AP (same clone as 18987-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 18987-1-AP (same clone as 18987-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 18987-1-AP (same clone as 18987-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 18987-1-AP (same clone as 18987-1-PBS)

  IP result of anti-DOCK9 (IP:18987-1-AP, 4ug; Detection:18987-1-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18987-1-AP (same clone as 18987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18987-1-AP (same clone as 18987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18987-1-AP (same clone as 18987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18987-1-AP (same clone as 18987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18987-1-AP (same clone as 18987-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK9 antibody (18987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18987-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18987-1-PBS cible DOCK9 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: dedicator of cytokinesis 9
• Masse moléculaire calculée: 239 kDa
• Poids moléculaire observé: 200-236 kDa
• Numéro d’acquisition GenBank: NM_015296
• Symbole du gène: DOCK9
• Identification du gène (NCBI): 23348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

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