Anticorps Polyclonal de lapin anti-DOT1L

DOT1L Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28145-1-PBS

Synonymes

Lysine N methyltransferase 4, KMT4, KIAA1814, H3 K79 HMTase, DOT1 like protein

---

Galerie de données de validation

• 

  WB analysis using 28145-1-AP (same clone as 28145-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28145-1-AP (DOT1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28145-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

28145-1-PBS cible DOT1L dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DOT1L Protéine recombinante Ag27229
• Nom complet: DOT1-like, histone H3 methyltransferase (S. cerevisiae)
• Masse moléculaire calculée: 185 kDa
• Poids moléculaire observé: 185~200 kDa
• Numéro d’acquisition GenBank: NM_032482
• Symbole du gène: DOT1L
• Identification du gène (NCBI): 84444
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DOT1L (Disruptor of telomeric silencing 1-like) is the only known histone methyltransferse catalyzing H3K79 methylation which is an activating mark for gene transcription by promoting transcriptional elongation (PMID: 34187895). DOT1L forms a complex with AF10/AF17 and ENL/AF9, is dysregulated in mixed-lineage leukemia (MLLr), and is believed to regulate transcriptional elongation without direct evidence. DOT1L has been reported to directly interact with RNAPII phosphorylated on Ser2 and/or Ser5 of the C-terminal domain (CTD) of its largest subunit. Loss of Dot1L results in yolk sac angiogenesis defects and embryonic lethality (PMID: 18787701).

{{ptg:RelatedPrimaryAntibodies}}