Anticorps Polyclonal de lapin anti-DPF2

• Phare
• Validé par KD/KO

DPF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 12111-1-AP

Synonymes

BAF45D, BRG1 associated factor 45D, BRG1-associated factor 45D, Protein requiem, REQ

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Galerie de données de validation

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  WB analysis using 12111-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12111-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12111-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12111-1-AP

  IP result of anti-DPF2 (IP:12111-1-AP, 4ug; Detection:12111-1-AP 1:6000) with Jurkat cells lysate 1600 ug.

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  IHC staining of human lung cancer using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary adenoma using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary adenoma using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12111-1-AP (DPF2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12111-1-AP (DPF2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12111-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DPF2 antibody (12111-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 12111-1-AP

  Immunofluorescent analysis of Hela cells, using DPF2 antibody 12111-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules K-562, cellules Y79, tissu de thymus de souris
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu d'adénome hypophysaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Informations sur le produit

12111-1-AP cible DPF2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DPF2 Protéine recombinante Ag2756
• Nom complet: D4, zinc and double PHD fingers family 2
• Masse moléculaire calculée: 391 aa, 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC014889
• Symbole du gène: DPF2
• Identification du gène (NCBI): 5977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DPF2, also named as BAF45D or UBID4, is a 391 amino acid protein, which belongs to the requiem/DPF family. DPF2 may be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells and might also have a role in the development and maturation of lymphoid cells. DPF2 was recently shown to inhibit the myeloid differentiation of hematopoietic stem/progenitor and acute myelogenous leukemia cells.

Publications

Species	Application	Title
mouse	IF	Nat Commun; SS18 regulates pluripotent-somatic transition through phase separation.; Authors - Junqi Kuang
human	WB	Anal Chem; Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling.; Authors - Yepei Huang
human	IHC	J Cancer; DPF2 overexpression correlates with immune infiltration and dismal prognosis in hepatocellular carcinoma; Authors - Kejian Yang
human	WB,IHC,IF	Front Neurosci; BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.; Authors - Zhenzhen Wang ; KD Validated
mouse,human	WB,IHC,IF	Front Neuroanat; The BAF45D Protein Is Preferentially Expressed in Adult Neurogenic Zones and in Neurons and May Be Required for Retinoid Acid Induced PAX6 Expression.; Authors - Chao Liu ; KD Validated
human	WB	Biosci Biotechnol Biochem; BAF45D knockdown decreases cell viability, inhibits colony formation, induces cell apoptosis and S-phase arrest in human pancreatic cancer cells.; Authors - Zengyi Liu ; KD Validated

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