Anticorps Polyclonal de lapin anti-DPH2

DPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12367-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human brain using 12367-1-AP (same clone as 12367-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  WB analysis using 12367-1-AP (same clone as 12367-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 12367-1-AP (same clone as 12367-1-PBS)

  IP result of anti-DPH2 (IP:12367-1-AP, 3ug; Detection:12367-1-AP 1:1000) with mouse skeletal muscle tissue lysate 6000ug. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12367-1-AP (same clone as 12367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12367-1-AP (DPH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

  Immunofluorescent analysis of PC-3 cells using 12367-1-AP (DPH2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12367-1-PBS cible DPH2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DPH2 Protéine recombinante Ag3042
• Nom complet: DPH2 homolog (S. cerevisiae)
• Masse moléculaire calculée: 489 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC016956
• Symbole du gène: DPH2
• Identification du gène (NCBI): 1802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.

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