Anticorps Recombinant de lapin anti-DPP10

DPP10 Recombinant Antibody for WB, IP, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241446A5

N° de cat : 84127-2-PBS

Synonymes

241446A5, dipeptidyl peptidase 10, Dipeptidyl peptidase IV-related protein 3, Dipeptidyl peptidase X, Dipeptidyl peptidase-like protein 2

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Galerie de données de validation

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  WB analysis using 84127-2-RR (same clone as 84127-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01035-3

  HT-29 cell lysate was measured. The human DPP10 concentration of detected samples was determined to be 3,448.25 ng/mL (based on a 4.4 mg/mL extract load).

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  Sandwich ELISA standard curve of MP01035-3

  Sandwich ELISA standard curve of MP01035-3, Human DPP10 Recombinant Matched Antibody Pair - PBS only. 84127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3231. 84127-3-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

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  IP experiment of pig brain using 84127-2-RR (same clone as 84127-2-PBS)

  IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84127-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84127-2-PBS against Human DPP10 was performed. The affinity constant is 3.15 nM.

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  Affinity and Kinetic Characterization of 84127-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

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Informations sur le produit

84127-2-PBS cible DPP10 dans les applications de WB, IP, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DPP10 Protéine recombinante Ag3231
• Nom complet: dipeptidyl-peptidase 10
• Masse moléculaire calculée: 796 aa, 91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC030832
• Symbole du gène: DPP10
• Identification du gène (NCBI): 57628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.

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