Anticorps Recombinant de lapin anti-DPP3

• Phare
• Validé par KD/KO

DPP3 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241360D4

N° de cat : 84173-3-RR

Synonymes

241360D4, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase 3, Dipeptidyl peptidase III

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Galerie de données de validation

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  WB analysis using 84173-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84173-3-RR (DPP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84173-3-RR

  WB result of DPP3 antibody (84173-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPP3 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 84173-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84173-3-RR against Human DPP3 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84173-3-RR cible DPP3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DPP3 Protéine recombinante Ag1022
• Nom complet: dipeptidyl-peptidase 3
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC007221
• Symbole du gène: DPP3
• Identification du gène (NCBI): 10072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DPP3 (dipeptidyl-peptidase 3), also known as DPPIII, is one of the dipeptidyl aminopeptidases, that belongs to the peptidase M49 family. DPP3 is a metalloenzyme containing one Zn2+ ion in its active site. Furthermore, It was shown to have a novel zinc-binding motif ( HELLGH). DPP3 has been reported as an important enkephalin-degrading enzyme in the central nervous system, in human neutrophils. It has 3 isoforms produced by alternative splicing. Increased activity of DPP3 is associated with endometrial and ovarian cancers.

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