Anticorps Recombinant de lapin anti-DPP4/CD26

DPP4/CD26 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242045F5

N° de cat : 84606-6-RR

Synonymes

CD26, 242045F5, ADABP, ADCP 2, ADCP2

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Galerie de données de validation

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  WB analysis using 84606-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 84606-6-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 84606-6-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 84606-6-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HuH-7 using 84606-6-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HuH-7 cells using DPP4/CD26 antibody (84606-6-RR, Clone: 242045F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of PC-3 using 84606-6-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DPP4/CD26 antibody (84606-6-RR, Clone: 242045F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84606-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84606-6-RR against Human DPP4/CD26 were performed. The affinity constant is 1.59 nM.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules LNCaP, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HuH-7, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84606-6-RR cible DPP4/CD26 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DPP4/CD26 Protéine recombinante Eg0157
• Nom complet: dipeptidyl-peptidase 4
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC013329
• Symbole du gène: DPP4
• Identification du gène (NCBI): 1803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

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