Anticorps Polyclonal de lapin anti-CRMP4

• Phare
• Validé par KD/KO

CRMP4 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13661-1-AP

Synonymes

CRMP 4, CRMP4, dihydropyrimidinase like 3, DPYSL3, DRP 3, DRP3, LCRMP, ULIP, ULIP 1, ULIP1, Unc 33 like phosphoprotein 1

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Galerie de données de validation

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  WB analysis using 13661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13661-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13661-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13661-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13661-1-AP cible CRMP4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRMP4 Protéine recombinante Ag4557
• Nom complet: dihydropyrimidinase-like 3
• Masse moléculaire calculée: 570 aa, 62 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC039006
• Symbole du gène: DPYSL3
• Identification du gène (NCBI): 1809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DPYSL3(Dihydropyrimidinase-related protein 3) is also named as CRMP4, DRP3, ULIP, ULIP1 and belongs to the hydantoinase/dihydropyrimidinase subfamily.DPYSL3 is a developmentally regulated protein, strongly expressed in early embryonic post-mitotic neural cells, reaching a peak of expression around the first post-natal week. In the adult brain, DPYSL3 is present mainly in regions that retain neurogenesis.DPYSL3 protein is also found in synaptic sites at the adult neuromuscular junction where it may be involved in the maintenance of neuronal stability(PMID: 16135096).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Mol Neurobiol; Collapsin response mediator protein 5 (CRMP5) modulates susceptibility to chronic social defeat stress in mice.; Authors - Yu-Fen Lin
mouse	WB	Neuropharmacology; Collapsin response mediator protein 5 (CRMP5) causes social deficits and accelerates memory loss in an animal model of Alzheimer's disease.; Authors - Yung-Shuen Lin
human	IF,IHC	Cancer Biol Ther; CRMP4a suppresses cell motility by sequestering RhoA activity in prostate cancer cells.; Authors - Changlin Li ; KD Validated
mouse	WB	Int J Mol Sci; Elevated Hippocampal CRMP5 Mediates Chronic Stress-Induced Cognitive Deficits by Disrupting Synaptic Plasticity, Hindering AMPAR Trafficking, and Triggering Cytokine Release; Authors - Yu-Fen Lin