Anticorps Polyclonal de lapin anti-DRD1

• Phare
• Validé par KD/KO

DRD1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 17934-1-AP

Synonymes

D(1A) dopamine receptor, DADR, Dopamine D1 receptor, dopamine receptor D1, DRD1, DRD1A

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Galerie de données de validation

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  WB analysis of mouse brain using 17934-1-AP

  37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 17934-1-AP (DRD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 17934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 17934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris incubé à 37 °C,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

17934-1-AP cible DRD1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DRD1 Protéine recombinante Ag12366
• Nom complet: dopamine receptor D1
• Masse moléculaire calculée: 446 aa, 49 kDa
• Poids moléculaire observé: 50-75 kDa
• Numéro d’acquisition GenBank: BC074978
• Symbole du gène: DRD1
• Identification du gène (NCBI): 1812
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dopamine is a neurotransmitter that plays a crucial role in physical and mental health, such as cardiovascular, hormonal, renal and central nervous systems (PMID: 29220802). Five subtypes of mammalian dopamine receptors are grouped into two classes, the D1- and D2-like classes. The D1-like class includes D1 and D5 receptors whereas the D2-like class includes D2, D3, D4 subtypes (PMID: 9457173). Dopamine receptor D1 (DRD1) is the most abundant form of dopamine receptor in the central nervous system. DRD1 stimulates adenylate cyclase, modulates D2 receptor activity, regulates neuron growth and differentiation, and mediates several behavioral responses (PMID: 1977312). DRD1 has a calculated molecular weight of 49 kDa, larger apparent molecular weight of 60-80 kDa may be due to glycosylation (PMID: 1281547; 23821371).

Publications

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
rat	WB	EMBO Mol Med; MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction.; Authors - Li Jingyuan J
human	IF	Elife; The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus.; Authors - Natasha M Puri
rat	WB	Int J Mol Sci; Effects of Maternal High-Fructose Diet on Long Non-Coding RNAs and Anxiety-like Behaviors in Offspring; Authors - Yuchen Zou
mouse	IF	CNS Neurosci Ther; Amphiregulin blockade decreases the levodopa-induced dyskinesia in a 6-hydroxydopamine Parkinson's disease mouse model; Authors - Piniel Alphayo Kambey
human	WB	Mol Neurobiol; Protective Effect of Curcumin by Modulating BDNF/DARPP32/CREB in Arsenic-Induced Alterations in Dopaminergic Signaling in Rat Corpus Striatum.; Authors - Pranay Srivastava

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