Anticorps Polyclonal de lapin anti-DRD5

• Phare
• Validé par KD/KO

DRD5 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20310-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20310-1-AP (same clone as 20310-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20310-1-AP (same clone as 20310-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20310-1-AP (same clone as 20310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20310-1-AP (same clone as 20310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 20310-1-AP (same clone as 20310-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug DRD5 Polyclonal antibody (20310-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20310-1-PBS cible DRD5 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DRD5 Protéine recombinante Ag14145
• Nom complet: dopamine receptor D5
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 50-53 kDa
• Numéro d’acquisition GenBank: BC009748
• Symbole du gène: DRD5
• Identification du gène (NCBI): 1816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DRD5, is a G-protein coupled receptor and involved in diversified physiological and pathological processes. this receptor stimulates adenylyl cyclase activity and is expressed in the limbic area of the mammalian brain(PMID:14699430). Studies have shown that DRD5 is highly expressed in colonic macrophages and DRD5 deficiency exacerbates DSS(dextran sodium sulfate)-induced colitis(PMID:34001860). in addition, DRD5 agonists were potent inhibitors of pituitary tumor growth(PMID:28613975).

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