Anticorps Polyclonal de lapin anti-DROSHA

• Phare
• Validé par KD/KO

DROSHA Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27958-1-PBS

Synonymes

RNASEN, EC:3.1.26.3, ETOHI2, HSA242976, p241

---

Galerie de données de validation

• 

  WB analysis using 27958-1-AP (same clone as 27958-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27958-1-AP (DROSHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 27958-1-AP (same clone as 27958-1-PBS)

  WB result of DROSHA antibody (27958-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DROSHA transfected HEK-293 cells. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

27958-1-PBS cible DROSHA dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DROSHA Protéine recombinante Ag27533
• Nom complet: ribonuclease type III, nuclear
• Masse moléculaire calculée: 159 aa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_001100412
• Symbole du gène: DROSHA
• Identification du gène (NCBI): 29102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.

{{ptg:RelatedPrimaryAntibodies}}