Anticorps Monoclonal anti-DSE

DSE Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C9F7

N° de cat : 60041-1-PBS

Synonymes

dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2

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Galerie de données de validation

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  WB analysis of COLO 320 using 60041-1-Ig (same clone as 60041-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60041-1-Ig (DSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60041-1-Ig (same clone as 60041-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSE antibody (60041-1-Ig, Clone: 2C9F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60041-1-PBS cible DSE dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DSE Protéine recombinante Ag0695
• Nom complet: dermatan sulfate epimerase
• Masse moléculaire calculée: 958 aa, 110 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC039245
• Symbole du gène: DSE
• Identification du gène (NCBI): 29940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

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