Anticorps Polyclonal de lapin anti-DSG1

DSG1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24587-1-PBS

Synonymes

Cadherin family member 4, CDHF4, desmoglein 1, Desmoglein-1, Desmosomal glycoprotein 1

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Galerie de données de validation

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  IHC staining of human skin cancer using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24587-1-AP (same clone as 24587-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSG1 antibody (24587-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24587-1-PBS cible DSG1 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DSG1 Protéine recombinante Ag20184
• Nom complet: desmoglein 1
• Masse moléculaire calculée: 1049 aa, 114 kDa
• Numéro d’acquisition GenBank: BC153001
• Symbole du gène: DSG1
• Identification du gène (NCBI): 1828
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).

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