Anticorps Recombinant de lapin anti-DSG1

DSG1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241093G11

N° de cat : 84053-6-PBS

Synonymes

Desmosomal glycoprotein 1, Desmoglein-1, desmoglein 1, CDHF4, Cadherin family member 4

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Galerie de données de validation

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  WB analysis using 84053-6-RR (same clone as 84053-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84053-6-RR (DSG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 84053-6-RR (same clone as 84053-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84053-6-RR (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 84053-6-RR (same clone as 84053-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84053-6-RR (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84053-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84053-6-RR against Human DSG1 were performed. The affinity constant is 71.2 pM.

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Informations sur le produit

84053-6-PBS cible DSG1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DSG1 Protéine recombinante Ag20184
• Nom complet: desmoglein 1
• Masse moléculaire calculée: 1049 aa, 114 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC153001
• Symbole du gène: DSG1
• Identification du gène (NCBI): 1828
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).

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