Anticorps Polyclonal de lapin anti-DSG2

• Phare
• Validé par KD/KO

DSG2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 21880-1-AP

Synonymes

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC

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Galerie de données de validation

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  WB analysis of HEK-293 using 21880-1-AP

  WB result of DSG2 antibody (21880-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DSG2 transfected HEK-293 cells.

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  WB analysis of HepG2 using 21880-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21880-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21880-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21880-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21880-1-AP

  WB result of DSG2 antibody (21880-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DSG2 transfected HeLa cells.

• 

  IP experiment of HepG2 using 21880-1-AP

  IP Result of anti-DSG2 (IP:21880-1-AP, 4ug; Detection:21880-1-AP 1:1000) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human lung cancer using 21880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21880-1-AP (DSG2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21880-1-AP (DSG2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 21880-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DSG2 antibody (21880-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 21880-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 21880-1-AP (DSG2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules A549, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

21880-1-AP cible DSG2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DSG2 Protéine recombinante Ag16393
• Nom complet: desmoglein 2
• Masse moléculaire calculée: 1118 aa, 122 kDa
• Poids moléculaire observé: 145-150 kDa
• Numéro d’acquisition GenBank: BC099655
• Symbole du gène: DSG2
• Identification du gène (NCBI): 1829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

Publications

Species	Application	Title
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
human	WB	Virol Sin; Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.; Authors - Jing Zhang ; KD Validated
human	IF	Helicobacter; Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.; Authors - Chen Zhang
human	IF	Mol Biol Cell; Plakophilin 3 and Par3 Facilitate Desmosomes' association with the Apical Junctional Complex.; Authors - Indrajyoti Indra
mouse	WB	Food Chem Toxicol; Sodium fluoride and sulfur dioxide affected male reproduction by disturbing blood-testis barrier in mice.; Authors - Jianhai Zhang
mouse	WB	Microscopy (Oxf); Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.; Authors - Shoko Ishikawa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Saba (Verified Customer) (09-26-2022)

it is said that this antibody is rabbit, however, unfortunately we observed a significant cross reaction with secondary anti mouse antibody.

• Applications: Immunofluorescence

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