Anticorps Monoclonal anti-DSP

DSP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10D4

N° de cat : 68364-1-PBS

Synonymes

desmoplakin, DP, DPI, DPII, DSP

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Galerie de données de validation

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  WB analysis using 68364-1-Ig (same clone as 68364-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68364-1-Ig (same clone as 68364-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68364-1-PBS cible DSP dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DSP Protéine recombinante Ag30499
• Nom complet: desmoplakin
• Masse moléculaire calculée: 2871 aa, 332 kDa
• Poids moléculaire observé: 240-330 kDa
• Numéro d’acquisition GenBank: BC140802
• Symbole du gène: DSP
• Identification du gène (NCBI): 1832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

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