Anticorps Polyclonal de lapin anti-DTL

DTL Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12896-1-AP

Synonymes

CDT2, CDW1, DCAF2, Denticleless protein homolog, DTL, L2DTL, RAMP

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Galerie de données de validation

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  WB analysis of HeLa using 12896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12896-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12896-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12896-1-AP

  IP Result of anti-DTL (IP:12896-1-AP, 3ug; Detection:12896-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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  IF Staining of HeLa using 12896-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12896-1-AP (DTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, cellules MCF-7
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12896-1-AP cible DTL dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DTL Protéine recombinante Ag3589
• Nom complet: denticleless homolog (Drosophila)
• Masse moléculaire calculée: 730 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC033540
• Symbole du gène: DTL
• Identification du gène (NCBI): 51514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

Publications

Species	Application	Title
mouse	IF	Sci Adv; Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.; Authors - Chunxia Zhang
human	WB	Proteomics; Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs; Authors - Chengli Yu