Anticorps Polyclonal de lapin anti-DTL

DTL Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12896-1-PBS

Synonymes

CDT2, CDW1, DCAF2, DDB1- and CUL4-associated factor 2, Denticleless protein homolog

---

Galerie de données de validation

• 

  WB analysis of HeLa using 12896-1-AP (same clone as 12896-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12896-1-AP (same clone as 12896-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 12896-1-AP (same clone as 12896-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 12896-1-AP (same clone as 12896-1-PBS)

  IP result of anti-DTL (IP:12896-1-AP, 3ug; Detection:12896-1-AP 1:500) with mouse testis tissue lysate 8000ug. This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12896-1-AP (same clone as 12896-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12896-1-AP (DTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

12896-1-PBS cible DTL dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DTL Protéine recombinante Ag3589
• Nom complet: denticleless homolog (Drosophila)
• Masse moléculaire calculée: 730 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC033540
• Symbole du gène: DTL
• Identification du gène (NCBI): 51514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

{{ptg:RelatedPrimaryAntibodies}}