Anticorps Polyclonal de lapin anti-Dysbindin

Dysbindin Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11132-1-AP

Synonymes

DBND, DTNBP1, DTNBP1a, DTNBP1b, Dysbindin, Dysbindin 1, dystrobrevin binding protein 1, HPS7, HPS7 protein, My031, SDY

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Galerie de données de validation

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  WB analysis of mouse brain using 11132-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11132-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11132-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

11132-1-AP cible Dysbindin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dysbindin Protéine recombinante Ag1613
• Nom complet: dystrobrevin binding protein 1
• Masse moléculaire calculée: 351 aa, 39 kDa
• Poids moléculaire observé: 48 kDa, 36 kDa
• Numéro d’acquisition GenBank: BC011912
• Symbole du gène: DTNBP1
• Identification du gène (NCBI): 84062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dysbindin, encoded by DTNBP1 (dystrobrevin binding protein 1), is a synaptic protein which is expressed in diverse neuronal populations throughout the brain. Genetic variation in DTNBP1 is associated with schizophrenia. Reduced expression of dysbindin has been reported in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia cases. Several isoforms of dysbindin exist: 48-50 kDa of dysbindin-1A, 35-38 kDa of dysbindin-1B, and 30-32 kDa of dysbindin-1C. This antibody recognizes all of isoforms of dysbindin. (19617633)

Publications

Species	Application	Title
		J Cell Biol; BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.; Authors - William J Monis
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook
mouse	WB	Neuropharmacology; Mice with dopaminergic neuron-specific deletion of DTNBP-1 gene show blunted nucleus accumbens dopamine release and associated behaviors.; Authors - Sanjeev K Bhardwaj
human	WB	Neuroscience; Protein kinase B/Akt1 phosphorylates dysbindin-1A at serine 10 to regulate neuronal development.; Authors - Erkang Fei
human	WB	Br J Pharmacol; Dopaminergic signaling and behavioral alterations by Comt-Dtnbp1 genetic interaction and their clinical relevance; Authors - Francesca Managò
mouse	WB,IF	Transl Psychiatry; Deletion of Dtnbp1 in mice impairs threat memory consolidation and is associated with enhanced inhibitory drive in the amygdala.; Authors - Cathy C Y Huang