Anticorps Polyclonal de lapin anti-DTX4

• Phare
• Validé par KD/KO

DTX4 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 25222-1-AP

Synonymes

Protein deltex 4, KIAA0937, EC:2.3.2.27, E3 ubiquitin-protein ligase DTX4, Deltex4

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Galerie de données de validation

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  WB analysis of mouse kidney using 25222-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25222-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse embryo using 25222-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25222-1-AP (DTX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 25222-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25222-1-AP (DTX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris
• Résultats positifs en IF-P: tissu embryonnaire de souris,
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

25222-1-AP cible DTX4 dans les applications de WB, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DTX4 Protéine recombinante Ag18415
• Nom complet: deltex homolog 4 (Drosophila)
• Masse moléculaire calculée: 619 aa, 67 kDa
• Poids moléculaire observé: 67-70 kDa
• Numéro d’acquisition GenBank: BC119011
• Symbole du gène: DTX4
• Identification du gène (NCBI): 23220
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DTX4, also named as KIAA0937, RNF155, is a 619 amino acid protein, which belongs to the Deltex family. DTX4 as a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. DTX4 functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.

Publications

Species	Application	Title
human	WB	Cancer Sci; Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.; Authors - Xin-Yu Ding
rat	WB	Stem Cells Dev; Combined Methylome and Transcriptome Analysis During Rat Hepatic Stellate Cell Activation.; Authors - Eva Christine Schumacher
mouse	WB,IF	Biochem Biophys Res Commun; E3 ubiquitin ligase DTX4 is required for adipogenic differentiation in 3T3-L1 preadipocytes cell line.; Authors - Zonggui Wang ; KD Validated
mouse	WB	Cell Death Dis; Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model; Authors - Jinxiang Wu

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