Anticorps Monoclonal anti-DUOX1

DUOX1 Monoclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3B8D6

Avis (1)

N° de cat : 67226-1-Ig

Synonymes

3B8D6, DUOX, DUOX 1, EC:1.11.1.-, Large NOX 1

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Galerie de données de validation

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  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67226-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse stomach using 67226-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using DUOX1 antibody (67226-1-Ig, Clone: 3B8D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat lung using 67226-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using DUOX1 antibody (67226-1-Ig, Clone: 3B8D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu placentaire humain
• Résultats positifs en IF-P: tissu d'estomac de souris, tissu pulmonaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67226-1-Ig cible DUOX1 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DUOX1 Protéine recombinante Ag18154
• Nom complet: dual oxidase 1
• Masse moléculaire calculée: 1551 aa, 177 kDa
• Poids moléculaire observé: 190-200 kDa, 177 kDa, 138 kDa
• Numéro d’acquisition GenBank: BC114938
• Symbole du gène: DUOX1
• Identification du gène (NCBI): 53905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DUOX1 (dual oxidase 1) is a calciumactivated transmembrane protein member of the NOX family of NADPH oxidases principally located on the apical surface of airway and thyroid epithelia (PMID: 28982074, 15677770, 10806195). DUOX1 is a strong generator of hydrogen peroxide (H2O2), via intra-molecular dismutation of superoxide (PMID: 25586178). Epithelial DUOX1 appears to participate in additional host defense actions, such as mucociliary clearance, and may contribute to inflammatory responses and epithelial repair processes during exogenous stress (PMID: 19386603). DUOX1 has two isofroms with the calculated molecular mass of 177 and 138 kDa, and apparent molecular mass of 180-200 kDa due to the glycosylation (PMID: 17643428, 19144650).

Publications

Species	Application	Title
human	IF	Cancers (Basel); Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.; Authors - Luc H J Sondorp
human	WB	BMC Mol Cell Biol; Upregulated dual oxidase 1-induced oxidative stress and caspase-1-dependent pyroptosis reflect the etiologies of heart failure; Authors - Yan Song Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Edma (Verified Customer) (03-03-2025)

The antibody is not that specific either in EB or in the IF images

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 (WB), 1:200 (IF)
• Cell Tissue Type: mouse lung homogenate (WB), mouse lungs (IF)

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