Anticorps Monoclonal anti-DUOX1

DUOX1 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3B8D6

N° de cat : 67226-1-Ig

Synonymes

dual oxidase 1, DUOX, DUOX1, Large NOX 1, LNOX1, Long NOX 1, NADPH thyroid oxidase 1, NOXEF1, THOX1, Thyroid oxidase 1

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Galerie de données de validation

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  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67226-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse stomach using 67226-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using DUOX1 antibody (67226-1-Ig, Clone: 3B8D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu placentaire humain
• Résultats positifs en IF: tissu d'estomac de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

67226-1-Ig cible DUOX1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DUOX1 Protéine recombinante Ag18154
• Nom complet: dual oxidase 1
• Masse moléculaire calculée: 1551 aa, 177 kDa
• Poids moléculaire observé: 190-200 kDa, 177 kDa, 138 kDa
• Numéro d’acquisition GenBank: BC114938
• Symbole du gène: DUOX1
• Identification du gène (NCBI): 53905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DUOX1 (dual oxidase 1) is a calciumactivated transmembrane protein member of the NOX family of NADPH oxidases principally located on the apical surface of airway and thyroid epithelia (PMID: 28982074, 15677770, 10806195). DUOX1 is a strong generator of hydrogen peroxide (H2O2), via intra-molecular dismutation of superoxide (PMID: 25586178). Epithelial DUOX1 appears to participate in additional host defense actions, such as mucociliary clearance, and may contribute to inflammatory responses and epithelial repair processes during exogenous stress (PMID: 19386603). DUOX1 has two isofroms with the calculated molecular mass of 177 and 138 kDa, and apparent molecular mass of 180-200 kDa due to the glycosylation (PMID: 17643428, 19144650).

Publications

Species	Application	Title
human	IF	Cancers (Basel); Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.; Authors - Luc H J Sondorp