Anticorps Recombinant de lapin anti-DUS2L

DUS2L Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240192C6

N° de cat : 83265-8-PBS

Synonymes

hDUS2, FLJ20399, EC:1.3.1.91, DUS2, Dihydrouridine synthase 2

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Galerie de données de validation

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  WB analysis using 83265-8-RR (same clone as 83265-8-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83265-8-RR (DUS2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83265-8-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83265-8-RR (same clone as 83265-8-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUS2L Recombinant antibody (83265-8-RR, Clone:240192C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83265-8-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83265-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83265-8-RR against Human DUS2L were performed. The affinity constant is 0.321 nM.

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Informations sur le produit

83265-8-PBS cible DUS2L dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DUS2L Protéine recombinante Ag23831
• Nom complet: dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC006527
• Symbole du gène: DUS2L
• Identification du gène (NCBI): 54920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Dihydrouridine synthase 2(DUS2L), is a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. transfection of DUS2 cDNA into a human lung carcinoma cell line increased dihydrouridine in tRNA, whereas transfection of DUS2 small interfering RNA (siRNA) reduced tRNA-dihydrouridine, confirming that DUS2 has dihydrouridine synthase activity.

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