Anticorps Polyclonal de lapin anti-DUSP11

• Phare
• Validé par KD/KO

DUSP11 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 10204-2-AP

Synonymes

RNA/RNP complex-1-interacting phosphatase, PIR1, EC:3.1.3.-, DUSP 11, Dual specificity protein phosphatase 11

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Galerie de données de validation

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  WB analysis using 10204-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10204-2-AP (DUSP11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10204-2-AP

  IP result of anti-DUSP11 (IP:10204-2-AP, 4ug; Detection:10204-2-AP 1:4000) with K-562 cells lysate 1760 ug.

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  IF Staining of A431 using 10204-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DUSP11 antibody (10204-2-AP) at dilution of 1:1400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:700-1:2800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below

Informations sur le produit

10204-2-AP cible DUSP11 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DUSP11 Protéine recombinante Ag0276
• Nom complet: dual specificity phosphatase 11 (RNA/RNP complex 1-interacting)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC000346
• Symbole du gène: DUSP11
• Identification du gène (NCBI): 8446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DUSP11, also known as PIR1, is a unique member of atypical DUSPs which could bind directly to RNA and possess RNA 5'-triphosphatase and diphosphatase activities. DUSP11 converts the 5' triphosphate of microRNA precursors to a 5' monophosphate, and regulates cellular noncoding RNAs levels. In addition to a catalysis towards RNA, more evidence showed that DUSP11 could also dephosphorylate proteins.

Publications

Species	Application	Title
human	WB	Ann Rheum Dis; Novel autoantibodies identified in ACPA-negative rheumatoid arthritis.; Authors - Ketian Li
human	WB	Nat Commun; RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection.; Authors - Yang Zhao
human	WB	Proc Natl Acad Sci U S A; RNA triphosphatase DUSP11 enables exonuclease XRN-mediated restriction of hepatitis C virus.; Authors - Rodney P Kincaid ; KO Validated
human	WB	Genes Dev; DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs.; Authors - James M Burke
human	WB	Virol Sin; HBV precore G1896A mutation promotes growth of hepatocellular carcinoma cells by activating ERK/MAPK pathway; Authors - Baoxin Zhao ; KD Validated
human	WB	RNA Biol; DUSP11 - an RNA phosphatase that regulates host and viral non-coding RNAs in mammalian cells.; Authors - James M Burke

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