Anticorps Polyclonal de lapin anti-DUSP2

DUSP2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 27327-1-AP

Synonymes

dual specificity phosphatase 2, DUSP2, PAC 1, PAC1

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Galerie de données de validation

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  WB analysis of A549 using 27327-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

27327-1-AP cible DUSP2 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DUSP2 Protéine recombinante Ag25380
• Nom complet: dual specificity phosphatase 2
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC007771
• Symbole du gène: DUSP2
• Identification du gène (NCBI): 1844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DUSP2 belongs to the sub-family of nuclear specific DUSPs that includes DUSP1, 4 and 5. DUSP2 is highly expressed in immune cells, in particular, of patients with inflammatory arthritis, such as in rheumatoid arthritis (RA), and Dusp2 knockout mice present a significant reduction in inflammatory responses. It has been demonstrated that DUSP2 is a downstream target of the tumor suppressor p53 that is required for the cellular response to oxidative stress. DUSP2 is also involved in transcription factor E2F1-mediated apoptotic signaling. Like other members of the DUSP family, DUSP2 interacts with MAPKs; However, the target protein for its phosphatase activity is controversial.

Publications

Species	Application	Title
human	WB	Hum Cell; Exosomal hsa_circ_0017252 attenuates the development of gastric cancer via inhibiting macrophage M2 polarization.; Authors - Jin Song
mouse	WB	Basic Clin Pharmacol Toxicol; Apatinib combined with Keytruda treatment induces apoptosis of gastric carcinoma cells through CES4/miR-616-5p/DUSP2 axis.; Authors - Fengli Zhang
human	FC	Blood Coagul Fibrinolysis; Effects of Momordica charantia exosomes on platelet activation, adhesion, and aggregation.; Authors - Jianchao Zhang
mouse	WB	Bioact Mater; Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity; Authors - Teng Ye
mouse	WB	Cell Rep; GABA regulates IL-1β production in macrophages; Authors - Jian Fu ; KD Validated