Anticorps Recombinant de lapin anti-DUSP6

DUSP6 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240295C8

Avis (1)

N° de cat : 83356-5-RR

Synonymes

MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6

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Galerie de données de validation

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  WB analysis using 83356-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83356-5-RR (DUSP6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83356-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of PC-12 using 83356-5-RR

  1x10^6 PC-12 cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83356-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83356-5-RR against Human DUSP6 were performed. The affinity constant is 3.38 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HepG2, cellules NIH/3T3, cellules PC-12, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en FC (Intra): cellules HeLa, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83356-5-RR cible DUSP6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DUSP6 Protéine recombinante Ag23474
• Nom complet: dual specificity phosphatase 6
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC003562
• Symbole du gène: DUSP6
• Identification du gène (NCBI): 1848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-30-2025)

DUSP6 (in green) worked very well in human FFPE brain tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

DUSP6 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:83356-5-RR)

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