Anticorps Polyclonal de lapin anti-DUSP9

DUSP9 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 26718-1-AP

Synonymes

dual specificity phosphatase 9, EC:3.1.3.16, MAP kinase phosphatase 4, Mitogen-activated protein kinase phosphatase 4, MKP 4

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Galerie de données de validation

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  WB analysis using 26718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26718-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 26718-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26718-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

26718-1-AP cible DUSP9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DUSP9 Protéine recombinante Ag25167
• Nom complet: dual specificity phosphatase 9
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC060837
• Symbole du gène: DUSP9
• Identification du gène (NCBI): 1852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dual specificity protein phosphatase 9 (DUSP9) is also named itogen-activated protein kinase phosphatase 4 (MAP kinase phosphatase 4; MKP-4). DUSP9 is a typical DUSP characterized by the presence of an MKB/KIM motif and a phosphatase domain, which shares structural homology with other DUSPs (PMID: 25519881). DUSP9 is an important downstream regulator of BMP/Smad signaling and a key factor in maintaining the stemness of mESCs (PMID: 34768967). DUSP9 is one of the key genes involved in gonadotrophin-mediated ovarian follicle development (PMID: 33676987). DUSP9 expression increases during insulin-induced adipogenesis and culminates in mature adipocytes. DUSP9 as a key regulator of insulin signaling and highlighted its potential role in insulin resistance and metabolic diseases by dephosphorylating kinases involved in metabolic processes, glucose uptake and storage. DUSP9 can also impair the action of extracellular mediators and stress inducers (i.e., proinflammatory cytokines), which can induce insulin resistance by abnormally activating MAPK or SAP pathways (PMID: 30063256, PMID: 18296638). In normal mature tissues, DUSP9 is mainly expressed in kidney, adipose tissue and placenta, while it is only minimally present in brain, ovary, testis and urinary bladder (PMID: 34768967).

Publications

Species	Application	Title
human	IHC	Front Immunol; Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma; Authors - Xiang-Xu Wang
mouse	WB	Sci Rep; Inhibition of miR-194-5p avoids DUSP9 downregulation thus limiting sepsis-induced cardiomyopathy; Authors - Jie Wang ; KD Validated
human	WB,IF	Adv Sci (Weinh); Malignant Hepatoblast-Like Cells Sustain Stemness via IGF2-Dependent Cholesterol Accumulation in Hepatoblastoma; Authors - Miao Ding

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