Anticorps Polyclonal de lapin anti-DVL1

• Phare
• Validé par KD/KO

DVL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27384-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 27384-1-AP (same clone as 27384-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 27384-1-AP (same clone as 27384-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 27384-1-AP (same clone as 27384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DVL1 antibody (27384-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27384-1-PBS cible DVL1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DVL1 Protéine recombinante Ag26055
• Nom complet: dishevelled, dsh homolog 1 (Drosophila)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 75 kDa, 73 kDa
• Numéro d’acquisition GenBank: BC050454
• Symbole du gène: DVL1
• Identification du gène (NCBI): 1855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

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