Anticorps Monoclonal anti-DVL2

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2F2D2

N° de cat : 67105-1-Ig

Synonymes

Dishevelled 2, 2F2D2, Dishevelled-2, DSH homolog 2, Segment polarity protein dishevelled homolog DVL-2

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Galerie de données de validation

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  WB analysis using 67105-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67105-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67105-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 67105-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules T-47D, cellules THP-1
• Résultats positifs en IHC: tissu de côlon de souris, tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de côlon de rat, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

67105-1-Ig cible DVL2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DVL2 Protéine recombinante Ag28634
• Nom complet: dishevelled, dsh homolog 2 (Drosophila)
• Masse moléculaire calculée: 736 aa, 79 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC014844
• Symbole du gène: DVL2
• Identification du gène (NCBI): 1856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IP	Int J Biol Sci; CYR61 Acts as an Intracellular Microtubule-Associated Protein and Coordinates Mitotic Progression via PLK1-FBW7 Pathway; Authors - Kaishun Hu
rat	WB	Biochim Biophys Acta Mol Cell Res; Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB; Authors - Weilin Fang
human	WB	bioRxiv; Wnt specifically induces FZD5/8 endocytosis and degradation and the involvement of RSPO-ZNRF3/RNF43 and DVL; Authors - Dong Luo

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