Anticorps Polyclonal de lapin anti-DVL3

DVL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13444-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13444-1-AP (same clone as 13444-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 13444-1-AP (same clone as 13444-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 13444-1-AP (same clone as 13444-1-PBS)

  IP result of anti-DVL3 (IP:13444-1-AP, 4ug; Detection:13444-1-AP 1:500) with Raji cells lysate 1440ug. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13444-1-AP (same clone as 13444-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DVL3 antibody (13444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13444-1-AP (same clone as 13444-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13444-1-AP (DVL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13444-1-PBS cible DVL3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DVL3 Protéine recombinante Ag4272
• Nom complet: dishevelled, dsh homolog 3 (Drosophila)
• Masse moléculaire calculée: 716 aa, 78 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC032459
• Symbole du gène: DVL3
• Identification du gène (NCBI): 1857
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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