Anticorps Recombinant de lapin anti-DYKDDDDK tag

DYKDDDDK tag Recombinant Antibody for IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4K14

N° de cat : 80010-1-PBS

Synonymes

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

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Galerie de données de validation

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  IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 4ug; Detection:80010-1-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug. This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DDDDK tag antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DYKDDDDK tag antibody), at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFP (green). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (80010-1-RR, Clone:4K14) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80010-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80010-1-RR against DYKDDDDK tag were performed. The affinity constant is 83.7 pM.

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Informations sur le produit

80010-1-PBS cible DYKDDDDK tag dans les applications de IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DYKDDDDK tag Protéine recombinante Ag2329
• Nom complet: DYKDDDDK tag
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

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