Anticorps Monoclonal anti-DYKDDDDK tag

DYKDDDDK tag Monoclonal Antibody for IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Protéine recombinante

Applications

IF/ICC, ELISA

Conjugaison

Biotin

CloneNo.

8H6A10

N° de cat : Biotin-66008-4

Synonymes

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

---

Galerie de données de validation

• 

  IF Staining of Transfected HEK-293 using Biotin-66008-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using Biotin DYKDDDDK tag antibody (Biotin-66008-4, Clone: 8H6A10 ) at dilution of 1:600 and Alexa Fluor 488 Streptavidin.

• 

  ELISA experiment of FusionProtein using Biotin-66008-4

  Ag2329 (DYKDDDDK tagged protein) was coated onto microtiter plates at 0.073 µg/well and then incubated with a dilution series of Biotin-66008-4 (start concentration 500 ng/ml). And then detected with Streptavidin Poly-HRP (1:2000) followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm. Negative control is 66008-4-Ig.

---

Applications testées

• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,
• Résultats positifs en ELISA: FusionProtein

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Test immuno-enzymatique (ELISA): ELISA : 1:2000-1:4000000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-66008-4 cible DYKDDDDK tag dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DYKDDDDK tag
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

{{ptg:RelatedPrimaryAntibodies}}