Anticorps Recombinant de lapin anti-DYKDDDDK tag

DYKDDDDK tag Recombinant Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante et plus (1)

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(2)

CloneNo.

4K14

N° de cat : CL488-80010

Synonymes

Flag-tag, Flag tag, DYKDDDDK, DDDDK tag

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Galerie de données de validation

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  IF Staining of Transfected HEK-293 using CL488-80010

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 488 DYKDDDDK tag antibody (CL488-80010, Clone: 4K14 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

CL488-80010 cible DYKDDDDK tag dans les applications de IF/ICC et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DYKDDDDK tag Protéine recombinante Ag2329
• Nom complet: DYKDDDDK tag
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publications

Species	Application	Title
Human	IF	Signal Transduct Target Ther; Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells; Authors - Pengju Wang
	IF	Theranostics; Designing multifunctional recombinant vaccines: an engineering strategy based on innovative epitope prediction-guided splicing; Authors - Zhidong Wang

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