Anticorps Polyclonal de lapin anti-DYNC1I2

• Phare
• Validé par KD/KO

DYNC1I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12219-1-PBS

Synonymes

IC2, DNCIC2, DNCI2, DH IC-2, DH IC 2

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Galerie de données de validation

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  WB analysis of Jurkat using 12219-1-AP (same clone as 12219-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12219-1-AP (same clone as 12219-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 12219-1-AP (same clone as 12219-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)

  IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12219-1-AP (same clone as 12219-1-PBS)

  IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 12219-1-AP (same clone as 12219-1-PBS)

  Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12219-1-PBS cible DYNC1I2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DYNC1I2 Protéine recombinante Ag2873
• Nom complet: dynein, cytoplasmic 1, intermediate chain 2
• Masse moléculaire calculée: 612 aa, 68 kDa
• Poids moléculaire observé: 68-72 kDa
• Numéro d’acquisition GenBank: BC015038
• Symbole du gène: DYNC1I2
• Identification du gène (NCBI): 1781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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