Anticorps Polyclonal de lapin anti-DYNLT1

• Phare
• Validé par KD/KO

DYNLT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 11954-1-AP

Synonymes

TCTEL1, T-complex testis-specific protein 1 homolog, Protein CW-1, Protein CW 1, Dynein light chain Tctex-type 1

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Galerie de données de validation

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  WB analysis of human brain using 11954-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11954-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Sp2/0 using 11954-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 11954-1-AP

  IP result of anti-DYNLT1 (IP:11954-1-AP, 4ug; Detection:11954-1-AP 1:300) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human pancreas using 11954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11954-1-AP (DYNLT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 11954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11954-1-AP (DYNLT1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse testis using 11954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DYNLT1 antibody (11954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 11954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DYNLT1 antibody (11954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 11954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DYNLT1 antibody (11954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules Sp2/0, tissu cardiaque humain
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

11954-1-AP cible DYNLT1 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DYNLT1 Protéine recombinante Ag2557
• Nom complet: dynein, light chain, Tctex-type 1
• Masse moléculaire calculée: 113 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC029412
• Symbole du gène: DYNLT1
• Identification du gène (NCBI): 6993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (Pubmed: 19448628)

Publications

Species	Application	Title
	IF	Nanomicro Lett; Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling; Authors - Junji Ren
mouse	WB,IHC,IF	Nat Neurosci; Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells.; Authors - Gauthier-Fisher Andrée A ; KD Validated
mouse	WB	Cell Res; Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein.; Authors - Kong Shanshan S
human	WB	J Cell Biol; Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.; Authors - Hironori Inaba ; KD Validated
human	IHC	Cancers (Basel); Dynein Light Chain Protein Tctex1: A Novel Prognostic Marker and Molecular Mediator in Glioblastoma.; Authors - Claudia Alexandra Dumitru
	WB	Biochem Pharmacol; Cannabinoid receptor-2 expression modulates Gβ1γ2 protein interaction with the activator of G protein signalling 2/dynein light chain protein Tctex-1.; Authors - Marina Nagler

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