Anticorps Monoclonal anti-DYNLT1

DYNLT1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2E3D4

N° de cat : 68312-1-Ig

Synonymes

CW 1, DYNLT1, Protein CW 1, TCTEL1, tctex 1, TCTEX1

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Galerie de données de validation

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  WB analysis using 68312-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68312-1-Ig (DYNLT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of C2C12 using 68312-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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  IF Staining of H9C2 using 68312-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68312-1-Ig cible DYNLT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DYNLT1 Protéine recombinante Ag33072
• Nom complet: dynein, light chain, Tctex-type 1
• Masse moléculaire calculée: 113 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC029412
• Symbole du gène: DYNLT1
• Identification du gène (NCBI): 6993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (PMID: 19448628)

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