Anticorps Polyclonal de lapin anti-DYX1C1

• Phare
• Validé par KD/KO

DYX1C1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14522-1-AP

Synonymes

DYX1, DYX1C1, DYXC1, EKN1, RD

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Galerie de données de validation

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  WB analysis using 14522-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14522-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 14522-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14522-1-AP

  IP result of anti-DYX1C1(IP:14522-1-AP, 4ug; Detection:14522-1-AP 1:800) with mouse testis tissue lysate 1120 ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14522-1-AP cible DYX1C1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DYX1C1 Protéine recombinante Ag5997
• Nom complet: dyslexia susceptibility 1 candidate 1
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC062564
• Symbole du gène: DYX1C1
• Identification du gène (NCBI): 161582
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYX1C1 initially was described as the first candidate gene for developmental dyslexia. The protein contains three TPR motifs and one p23 domain, all commonly known for their protein-protein interactions. It also has been linked to neuronal migration. Recently it has been reported that the expression of DYX1C1 protein is lost in breast cancer and patients who have lost DYX1C1 expression have a poor overall survival,indicating that DYX1C1 is a potential prognostic biomarker in breast cancer.

Publications

Species	Application	Title
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A ; KO Validated
human	IF	Biol Psychiatry; Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins.; Authors - Kristiina Tammimies
human	WB,IF	Mol Endocrinol; The rs3743205 SNP is important for the regulation of the dyslexia candidate gene DYX1C1 by estrogen receptor β and DNA methylation.; Authors - Tammimies Kristiina K
human	IHC	BMC Cancer; The dyslexia candidate gene DYX1C1 is a potential marker of poor survival in breast cancer.; Authors - Rosin Gustaf G
human	WB,IP	BMC Mol Cell Biol; Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2; Authors - Andrea Bieder